L-band (3.5 micron) IR-excess in massive star formation, II. RCW 57/NGC 3576

We present a JHKL survey of the massive star forming region RCW 57 (NGC 3576) based on L-band data at 3.5 micron taken with SPIREX (South Pole Infrared Explorer), and 2MASS JHK data at 1.25-2.2 micron. This is the second of two papers, the first one concerning a similar JHKL survey of 30 Doradus. Colour-colour and colour-magnitude diagrams are used to detect sources with infrared excess. This excess emission is interpreted as coming from circumstellar disks, and hence gives the cluster disk fraction (CDF). Based on the CDF and the age of RCW 57, it is possible to draw conclusions on the formation and early evolution of massive stars. The infrared excess is detected by comparing the locations of sources in JHKL colour-colour and L vs. (K-L) colour-magnitude diagrams to the reddening band due to interstellar extinction. A total of 251 sources were detected. More than 50% of the 209 sources included in the diagrams have an infrared excess. Comparison with other JHKL surveys, including the results on 30 Doradus from the first paper, support a very high initial disk fraction (>80%) even for massive stars, although there is an indication of a possible faster evolution of circumstellar disks around high mass stars. 33 sources only found in the L-band indicate the presence of heavily embedded, massive Class I protostars. We also report the detection of diffuse PAHs emission throughout the RCW 57 region.Comment: 15 pages, 13 figure

Unlocking the Keyhole - H2 and PAH emission from molecular clumps in the Keyhole Nebula

To better understand the environment surrounding CO emission clumps in the Keyhole Nebula, we have made images of the region in H2 1-0 S(1) (2.122 um) emission and polycyclic aromatic hydrocarbon (PAH) emission at 3.29 um. Our results show that the H2 and PAH emission regions are morphologically similar, existing as several clumps, all of which correspond to CO emission clumps and dark optical features. The emission confirms the existence of photodissociation regions (PDRs) on the surface of the clumps. By comparing the velocity range of the CO emission with the optical appearance of the H2 and PAH emission, we present a model of the Keyhole Nebula in which the most negative velocity clumps are in front of the ionization region, the clumps at intermediate velocities are in it, and those which have the least negative velocities are at the far side. It may be that these clumps, which appear to have been swept up from molecular gas by the stellar winds from eta Car, are now being over-run by the ionization region and forming PDRs on their surfaces. These clumps comprise the last remnants of the ambient molecular cloud around eta Car.Comment: 8 pages, 4 figures, to be published in MNRA

Photodissociation regions and star formation in the Carina Nebula

We have obtained wide-field thermal infrared (IR) images of the Carina Nebula, using the SPIREX/Abu telescope at the South Pole. Emission from poly-cyclic aromatic hydrocarbons (PAHs) at 3.29um, a tracer of photodissociation regions (PDRs), reveals many interesting well defined clumps and diffuse regions throughout the complex. Near-IR images (1--2um), along with images from the Midcourse Space Experiment (MSX) satellite (8--21um) were incorporated to study the interactions between the young stars and the surrounding molecular cloud in more detail. Two new PAH emission clumps have been identified in the Keyhole Nebula and were mapped in 12CO(2--1) and (1--0) using the SEST. Analysis of their physical properties reveals they are dense molecular clumps, externally heated with PDRs on their surfaces and supported by external pressure in a similar manner to the other clumps in the region. A previously identified externally heated globule containing IRAS 10430-5931 in the southern molecular cloud, shows strong 3.29-, 8- and 21-um emission, the spectral energy distribution (SED) revealing the location of an ultra-compact (UC) HII region. The northern part of the nebula is complicated, with PAH emission inter-mixed with mid-IR dust continuum emission. Several point sources are located here and through a two-component black-body fit to their SEDs, we have identified 3 possible UC HII regions as well as a young star surrounded by a circumstellar disc. This implies that star formation in this region is on-going and not halted by the intense radiation from the surrounding young massive stars.Comment: 14 pages, 12 figures. Accepted by MNRAS. Higher resolution figures available at http://www.phys.unsw.edu.au/~jmr/papers.htm

What is missing in autonomous discovery: Open challenges for the community

Self-driving labs (SDLs) leverage combinations of artificial intelligence, automation, and advanced computing to accelerate scientific discovery. The promise of this field has given rise to a rich community of passionate scientists, engineers, and social scientists, as evidenced by the development of the Acceleration Consortium and recent Accelerate Conference. Despite its strengths, this rapidly developing field presents numerous opportunities for growth, challenges to overcome, and potential risks of which to remain aware. This community perspective builds on a discourse instantiated during the first Accelerate Conference, and looks to the future of self-driving labs with a tempered optimism. Incorporating input from academia, government, and industry, we briefly describe the current status of self-driving labs, then turn our attention to barriers, opportunities, and a vision for what is possible. Our field is delivering solutions in technology and infrastructure, artificial intelligence and knowledge generation, and education and workforce development. In the spirit of community, we intend for this work to foster discussion and drive best practices as our field grows

A Federated Design for a Neurobiological Simulation Engine: The CBI Federated Software Architecture

Simulator interoperability and extensibility has become a growing requirement in computational biology. To address this, we have developed a federated software architecture. It is federated by its union of independent disparate systems under a single cohesive view, provides interoperability through its capability to communicate, execute programs, or transfer data among different independent applications, and supports extensibility by enabling simulator expansion or enhancement without the need for major changes to system infrastructure. Historically, simulator interoperability has relied on development of declarative markup languages such as the neuron modeling language NeuroML, while simulator extension typically occurred through modification of existing functionality. The software architecture we describe here allows for both these approaches. However, it is designed to support alternative paradigms of interoperability and extensibility through the provision of logical relationships and defined application programming interfaces. They allow any appropriately configured component or software application to be incorporated into a simulator. The architecture defines independent functional modules that run stand-alone. They are arranged in logical layers that naturally correspond to the occurrence of high-level data (biological concepts) versus low-level data (numerical values) and distinguish data from control functions. The modular nature of the architecture and its independence from a given technology facilitates communication about similar concepts and functions for both users and developers. It provides several advantages for multiple independent contributions to software development. Importantly, these include: (1) Reduction in complexity of individual simulator components when compared to the complexity of a complete simulator, (2) Documentation of individual components in terms of their inputs and outputs, (3) Easy removal or replacement of unnecessary or obsoleted components, (4) Stand-alone testing of components, and (5) Clear delineation of the development scope of new components

Science Programs for a 2 m-class Telescope at Dome C, Antarctica: PILOT, the Pathfinder for an International Large Optical Telescope

The cold, dry and stable air above the summits of the Antarctic plateau provides the best ground-based observing conditions from optical to sub-mm wavelengths to be found on the Earth. PILOT is a proposed 2 m telescope, to be built at Dome C in Antarctica, able to exploit these conditions for conducting astronomy at optical and infrared wavelengths. While PILOT is intended as a pathfinder towards the construction of future grand-design facilities, it will also be able to undertake a range of fundamental science investigations in its own right. This paper provides the performance specifications for PILOT, including its instrumentation. It then describes the kinds of science projects that it could best conduct. These range from planetary science to the search for other solar systems, from star formation within the Galaxy to the star formation history of the Universe, and from gravitational lensing caused by exo-planets to that produced by the cosmic web of dark matter. PILOT would be particularly powerful for wide-field imaging at infrared wavelengths, achieving near-diffraction limited performance with simple tip-tilt wavefront correction. PILOT would also be capable of near-diffraction limited performance in the optical wavebands, as well be able to open new wavebands for regular ground based observation; in the mid-IR from 17 to 40 microns and in the sub-mm at 200 microns.Comment: 74 pages, 14 figures, PASA, in pres

Diacylglycerol-Stimulated Endocytosis of Transferrin in Trypanosomatids Is Dependent on Tyrosine Kinase Activity

Small molecule regulation of cell function is an understudied area of trypanosomatid biology. In Trypanosoma brucei diacylglycerol (DAG) stimulates endocytosis of transferrin (Tf). However, it is not known whether other trypanosomatidae respond similarly to the lipid. Further, the biochemical pathways involved in DAG signaling to the endocytic system in T. brucei are unknown, as the parasite genome does not encode canonical DAG receptors (e.g. C1-domains). We established that DAG stimulates endocytosis of Tf in Leishmania major, and we evaluated possible effector enzymes in the pathway with multiple approaches. First, a heterologously expressed glycosylphosphatidylinositol phospholipase C (GPI-PLC) activated endocytosis of Tf 300% in L. major. Second, exogenous phorbol ester and DAGs promoted Tf endocytosis in L. major. In search of possible effectors of DAG signaling, we discovered a novel C1-like domain (i.e. C1_5) in trypanosomatids, and we identified protein Tyr kinases (PTKs) linked with C1_5 domains in T. brucei, T. cruzi, and L. major. Consequently, we hypothesized that trypanosome PTKs might be effector enzymes for DAG signaling. General uptake of Tf was reduced by inhibitors of either Ser/Thr or Tyr kinases. However, DAG-stimulated endocytosis of Tf was blocked only by an inhibitor of PTKs, in both T. brucei and L. major. We conclude that (i) DAG activates Tf endocytosis in L. major, and that (ii) PTKs are effectors of DAG-stimulated endocytosis of Tf in trypanosomatids. DAG-stimulated endocytosis of Tf may be a T. brucei adaptation to compete effectively with host cells for vertebrate Tf in blood, since DAG does not enhance endocytosis of Tf in human cells